qPCR 2005 Symposium
Proceedings ( ISBN 3000166874 ) |
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Session |
Speaker |
Title |
Keynote speaker |
Russell Higuchi "Pioneer in real-time PCR" Associate Director of the Human Genetics Department, Roche Molecular Systems, Almeda, CA, USA |
Real-time PCR, a personal
perspective. |
Pre-analytical steps |
Stephen A Bustin Professor of Molecular Science Institute of Cell and Molecular Science Queen Mary's School of Medicine and Dentistry, University of London |
mRNA
quantification from archival cancer samples. Normalisation of mRNA levels against total DNA content. |
Bioinformatics |
Mikael Kubista Professor of Biotechnology, MultiD Analyses AB, Göteborg, Sweden |
Classification of real-time
PCR data. |
New Applications |
Neven Zoric Coordinator of the TATAA Biocenter Göteborg, Sweden |
The best of both worlds -
New dyes for qPCR for use in combination with probes. |
Pre-analytical steps |
Michael W. Pfaffl Reader in Physiology Center of Life Science, Weihenstephan Technical University of Munich, Germany |
Influence of RNA matrix effect on qRT-PCR
results - an overview. |
Array Verification |
Elisa Wurmbach Assistant Professor Mount Sinai School of Medicine Department of Hematology/Oncology New York, NY, USA |
Correlation of microarray
and quantitative real-time RT-PCR results. |
Optimization |
Greg
Shipley Director, Quantitative Genomics Core Laboratory, The University of Texas Health Science Centre- Houston, USA |
Design
and optimization of Taqman and SYBR Green I real-time qPCR assays. Shipley-2-qPCR-2005.pdf |
Standardization |
Pamela Scott Adams Director, Molecular Biology Core Facility, Trudeau Institute, Saranac Lake, NY, USA |
A Comparison of Real-Time
RT-PCR Technique, Chemistries and Instrumentation in Laboratories
Utilizing the Same Assay. |
Normalization |
Jo Vandesompele Center for Medical Genetics Ghent Ghent University Hospital, Ghent, Belgium |
Normalization
of gene expression: state of the art and preview on a new strategy
using expressed Alu repeats. |
BioInformatics |
Jan Hellemans Center for Medical Genetics Ghent Ghent University Hospital, Ghent, Belgium |
qBase:
relative quantification software for management and automated analysis. |
New Applications |
Bernadett Papp Gene Expression Unit EMBL, Heidelberg, Germany |
ChIP experiments from Drosophila tissues
analyzed by qRT-PCR. |
Optimization |
Anders Stahlberg Department of Chemistry and Biosciences Chalmers University of Technology Göteborg, Sweden |
Single Cell Transcriptome. |
GMO |
Ron
Jenkins USDA/GIPSA USA |
The USDA/GIPSA Proficiency
Program: A Summary of Participants Capabilities for Detecting and
Quantifying Transgenic Events in Corn and Soybeans. |
Keynote speaker GMO |
Philipp Hübner Kantonales Laboratorium Basel-Stadt, Abteilungsleiter Lebensmittel, Basel, Switzerland |
Uncertainties and certainties in GMO
analytics using qPCR. |
GMO |
Isabel
Taverniers Ministry of the Flemish Community,Centre for Agricultural Research – CLO,Dept. for Plant Genetics and Breeding – DvP, Belgium |
Cloned plasmid DNA molecules as a tool
for GMO analysis. |
GMO |
Francisco Moreano Bayerisches Landesamt für Gesundheit und Lebensmittelsicherheit (LGL), Infektologie / Molekularbiologie, Germany |
Application of synthetic DNA-standards
for the quantitative screening of different genetically modified
rapeseed lines via real-time PCR. |
New
Applications |
Kristina
Lind Department of Chemistry and Biosciences Chalmers University of Technology Göteborg, Sweden |
Real-time immuno-PCR for
the quantification of proteins. |
Bioinformatics |
Tzachi Bar & Ales Tichopad LabonNet, Silberhornstrasse 3, Kirchheim bei München, Germany |
Efficiency estimation from
single amplification curve – methods and applications. |
Standardization |
Bob
Rutledge Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre Sainte-Foy, Quebec, Canada |
Putting the "quantity "
into
quantitative PCR: A simplified approach to the establishment and application of quantitative scale. |
Bioinformatics |
Bob
Rutledge |
Amplification efficiency
dynamics and its implications: Developing a kinetic-based approach for
quantitative analysis. |
Standardization |
Morten
T. Andersen Bio-Molecular Innovation, LGC Teddington, Middlesex, UK |
The Data Comparability Challenge
- Standards and Best Practices. |
New Applications |
Jim
Huggett Centre for Infectious Diseases & International Health, University College London, UK |
Infectious disease diagnostic
research in Africa; the role of real time PCR. |
New
Applications |
Arthur
Reis Brandeis University, Boston, MA, USA |
Forensic and Single-Molecule Assays
of Mitochondrial DNA Using LATE-PCR. |
Speaker |
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New
Applications |
Michael
Boutros Boveri-Group Signaling and Functional Genomics, DKFZ, Heidelberg, Germany sponsored by Roche Applied Science |
Rapid
Development of RT-PCR Assays for RNAi Experiments Using Pre-designed
LNA-probe Libraries. |
Optimization |
Raimund
Kinne Experimentelle Rheumatologie, Klinikum der Friedrich-Schiller-Universität Jena , Germany sponsored by Eppendorf |
Comparison
of MMP gene expression analysis by capillary and "realplex" real-time
PCR. |
Food Hygiene |
Martina
Fricker Dep. of Bioscience, Technical University of Munich, Freising, Germany sponsored by Cepheid |
Detection of Food Pathogens
using the Smart Cycler II. |
Pre-analytical steps |
James Willey Medical University of Ohio, TOLEDO, OH 43614, USA |
Use of
standardized mixtures of internal standards in
RT-PCR to generate validated biomarkers and to develop standardized
transcript abundance reference databases. |
Speaker |
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Standardization |
Oliver Geulen Roche Applied Science, Mannheim, Germany |
Accurate Gene Expression Analysis with High Flexibility: Concepts and Developments. |
Optimization |
Cynthia
Potter Eppendorf UK |
Validation of fast PCR protocols with the
Eppendorf Mastercycler ep realplex. |
Pre-analytical steps | Guido
Hennig Bayer HealthCare AG Diagnostics Research Germany Leverkusen |
Nucleic acid isolation for diagnostic
testing using Bayer´s magnetic particles. |
Optimization |
Olfert
Landt TIB MOLBIOL Syntheselabor GmbH Eresburgstraße, Berlin, Germany |
Finding the needle in the
haystack - LNA bases enhance SNP detection dramatically. |
GMO |
Dörte
Wulff Research and Development Eurofins Genescan / GeneScan Analytics GmbH, Freiburg, Germany |
Accurate
GMO quantification in food samples. |
New Applications |
Andreas
Missel Associate Director R&D, QIAGEN GmbH, Hilden |
Going MULTI – how to easily achieve high
multiplexing in real-time PCR. |
New
Applications |
Hilary
K. Srere Bio-Rad Laboratories Europe, Munich, Germany |
The Ups and Downs of Gene Regulation:
Validating siRNA Gene Expression Disruption with RT-qPCR. |
Pre-analytical steps |
L.
Scott Basehore Sr. Research Associate, Stratagene Research & Development Department |
Nucleic Acid Stabilization in Cultured Cell and Tissue Lysates for QPCR Gene Expression Analysis. |
New Applications |
Kyle
Hooper Promega Corporation, Woods Hollow Road, Madison, WI, USA |
Plexor™ Real-Time Quantitative PCR
Systems: Multiplexed assays made easy. |
Optimization |
Clemence
Beslin product manager, Eurogentec, Belgium |
qPCR
pitfalls - primer and probe design / fluorophore quencher combinations. |
Optimization |
Thomas
Kaiser Corbett Research, Sydney, Australia |
The fitness of a football
team: High Resolution Melts for the determination of genotypes. |
New-Applications |
Mark
Andersen Invitrogen, USA |
Two-color
multiplex assay for the identification of Orthopoxes viruses with
Real-Time LUX PCR. |
Transcriptomics |
Simone
Guenther Applied Biosystems, Applera Deutschland GmbH, Darmstadt, Germany |
microRNA expression
profiles from real-time PCR classify ES and differentiated cells. |
Bioinformatics |
Ben Sowers Research Associate, Biosearch Technologies |
From
Sequences to Synthesis: Optimal Amplification through Careful
Oligonucleotide Selection. |
Pre-analytical steps |
Marc
Valer Agilent Technologies, Waldbronn, Germany |
Standardization
of RNA Quality Assessment using the RNA Integrity Number (RIN) and the
2100 bioanalyzer. |
Standardization |
Andreas
Eckelt Cepheid SA, Germany |
Assay
standardisation using universal internal controls and lyophilized
reagent beads. |
All scientific
contributions
are published in the qPCR 2005 Symposium
Proceedings ISBN
3000166874
|