Description of REST-RG
beta software version 3 [ August 2006 ]
->
up
to 15 genes can
be analysed
-> up to 20
replicated per group
-> more reference genes can be chosen
-> calculation of an
geometric mean of the chosen RGs => RG
index
->
optimal for Rotor-Gene
3000 and 6000 applications
-> direct import of Rotor-Gene take
off points (TOP) via copy-and-paste
-> direct import of
single-run qPCR amplification
efficiencies via copy-and-paste
-> manual efficiency input
-> data output in a
graph with error bars
->
error estimation of the calculated ratio using a Taylor's series
-> bugs removed in
randomisation test
-> go to download
page
Page 1: Introduction and Gene name input
Page 2: TOP and PCR
efficiency input
Page 3: Copy
of TOP data input
Page 4: Variation
data output
Page 5: graphical output
ratio error estimation used on page 4 and graph
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