Description of REST-384 beta software version 2 [ August
2006 ]
New features
in REST-384:
-> up
to 15 genes can
be analysed
-> up to 20 replicated per group
-> more reference genes can be chosen
-> calculation
of an geometric mean of the chosen RGs => RG index
->
optimal for high throughput 96- and 384-well plate qPCR applications
-> efficiency calculation via
dilution
row
->
manual efficiency input
-> data output
in a graph with error bars
-> error estimation of the calculated ratio
using a Taylor's series
->
bugs removed in
randomisation test
-> go to download page
Page 1: Introduction and Gene name input
Page 2: PCR
efficiency calculation
Page 3:
Crossing Point ( CP or CT ) data input
Page 4: Variation
data output
Page 4: ratio error estimation
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