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real-time
PCR cyclers (3)
real-time PCR
cyclers (1)
real-time
PCR cyclers (2)
real-time
PCR cyclers (4)
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Mic
is a small 48-well qPCR instrument that
uses magnetic induction technology for rapid
real time cycling. Available with either 2 or 4
channels, the advantages of the Mic include:
• Speed – complete high
quality qPCR runs in less than 25 min.
• Accuracy – excellent
repeatability and reproducibility with a
temperature uniformity of ±0.05°C.
• Size – At 2 kg the Mic takes
up less space on a bench than a lab book.
• Connectivity – Bluetooth®
option allows up to 10 instruments to run from
one PC.
• Plug and play with no
calibration required – ever!
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Mic’s
user
friendly software is packed full of
intelligent analysis features including:
• Cycling analysis - with
LinRegPCR option (Ruijter et
al. 2009)
• Melt analysis -
including genotyping with dual hybridisation
probes
• Absolute Quantification
- including Standard Curve
• Relative Quantification
- incorporating the REST algorithm (Pfaffl et al. 2002)
• Allelic Discrimination
• High Resolution Melting
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| With an intuitive
software layout, run setup and analysis become
simple and there are many quality control
features to provide confidence in the final
result. The software has been designed to meet
the MIQE guidelines. |
| Discover
more at www.mic-qpcr.com
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Qiagen -
Rotor-Gene Q with HRM
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Real-time
PCR detection - Rotor-Gene
Q - pure detection
QIAGEN’s new real-time PCR cycler, the
Rotor-Gene Q, provides outstanding performance
and the reliable and accurate results your
research demands. Together with optimized
QIAGEN kits, the Rotor-Gene Q enables
streamlined analysis for a wide range of
applications.
* Outstanding thermal and
optical performance
* More applications than
any other real-time cycler
* Unmatched optical range
with up to 6 channels
* Robust design enables
easy assay setup and minimal maintenance
* Compatible with the
QIAgility for automated PCR setup
The unique rotary design
delivers:
* Well-to-well variations
below ±0.01°C (20 times lower than block
cyclers)
* Uniform detection
eliminating the need for ROX reference dye
* Fast ramping and
negligible equilibration times for short run
times
* Complete confidence in
your results!
Comprehensive application
range
The Rotor-Gene Q combined with optimized
QIAGEN real-time PCR kits and assays offer
reliable results in all real-time PCR and
high-resolution melt applications, including:
* Gene expression analysis
* Pathogen detection
* DNA methylation analysis
* Genotyping
* Mutation scanning
* High Resolution
Melting analysis
Rotor-Gene
ScreenClust HRM Software for highly
effective high-resolution melting analysis
* Innovative mathematical
approach to HRM analysis
* Highly accurate
identification of genotypes in supervised mode
* Automatic detection of
new mutations in unsupervised mode
* Robust statistics for
classifying and interpreting HRM data
* Minimal effort and
standardized processes for data interpretation
HRM
- an innovative technology for analysis of
genetic variations
View and listen to this seminar
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Principle and
procedure - Unique rotary design for
outstanding performance
The unique centrifugal rotary design of the
Rotor-Gene Q makes it the most precise and
versatile real-time PCR cycler currently
available (see figure "Cross-section of the
Rotor-Gene Q"). Each tube spins in a chamber
of moving air, keeping all samples at
precisely the same temperature during rapid
thermal cycling. Detection is similarly
uniform. When each tube aligns with the
detection optics, the sample is illuminated
and the fluorescent signal is rapidly
collected from a single, short optical
pathway. This thermal and optical uniformity
results in sensitive, precise, and fast
real-time PCR analysis (see figure "Precise
real-time PCR analysis"). It also eliminates
sample-to-sample variations and edge effects.
These are unavoidable in traditional
block-based instruments due to temperature
gradients across the block and multiple,
complex optical pathways.
The rotary design delivers:
* Well-to-well variation
below ±0.01°C (20 times less than block
cyclers)
* Uniform detection
eliminating the need for ROX reference dye
* Fast ramping and
negligible equilibration times for short run
times
* Complete confidence in
your results!
Unrivaled
optical range enables multiple applications
Whether your assay is based on intercalating
dyes such as SYBR Green, probes such as
hydrolysis (TaqMan) or hybridization (FRET)
probes, or multiplex chemistries, the
Rotor-Gene Q meets your requirements. With up
to 6 channels spanning UV to infrared
wavelengths, the cycler delivers the widest
optical range currently available (see Table
"Channels for optical detection"). In
addition, the software allows you to create
new excitation/detection wavelength
combinations, which means that the Rotor-Gene
Q is compatible with dyes you may use in the
future.
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REST 2009
Software
For gene expression analysis using real-time
PCR data from the Rotor-Gene Q and other
cyclers
* Estimates up and down
regulation for gene expression studies
* Analysis using
randomization and bootstrapping techniques
* Graphical data output via
whisker-box plots
REST 2009 Software is a standalone software
tool, developed by M. Pfaffl (Technical
University Munich) and QIAGEN, for analysis of
gene expression data from quantitative
real-time PCR experiments. Click the
"Resources" tab to download REST 2009 Software
free of charge.
Download
newest
version
REST 2009 =>
http://www.REST.de.com
Download User Guide =>
REST
2009
Software User Guide - English (PDF)
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The Rotor-Gene 6000 is a third-generation
real-time rotary analyzer. Its unique
centrifugal format was designed specifically
for the demanding requirements of real-time
cycling and thermo-optical analyses.
The rotary design provides unparalleled
well-to-well thermal and optical
uniformity—critical factors in real-time
analysis. Its also enables a fast data
acquisition rate, a feature now proving to be
essential for advanced applications such as
HRM (high-resolution melt).
In short, the superior performance of the
Rotor-Gene 6000 delivers versatility:
- It is the only real-time
amplification platform capable of HRM
(high-resolution melt) and nucleic acid
concentration measurement in addition to
all the standard real-time applications
like quantitative amplification and SNP
genotyping.
- It has the greatest optical range,
with 6 channels spanning UV to infra-red
wavelengths.
- It uses the widest range of
interchangeable reaction tube formats.
With the Rotor-Gene 6000 you can embrace
or invent the future of real-time analysis and
unlock new research possibilities. MORE… |
SmartCycler
II & GeneXpert (Cepheid)
 
The "heart" of the Smart Cycler®
System from Cepheid of Sunnyvale, Calif., is the
I-CORE™ (Intelligent Cooling/Heating Optical
Reaction) module. According to company literature,
the I-CORE module incorporates state-of-the-art
microfluidic and microelectronic design. Each
Smart Cycler processing block contains 16
independently programmable I-CORE modules, each of
which performs four-color, real-time fluorometric
detection. A wide variety of different multiplex
or simplex fluorescent tags can used in
conjunction with this system, including FAM,
TET, ALEXA 532, ALEXA 647,
TAM, ROX, SYBR Green, Cy3, Texas Red
and others.
Main features of the
SmartCycler II system:
• Expandable: 16, 32, 48,
64, 80 or 96 independent reaction sites
• Flexible
• Fast
• Real-time detection
• Four-channel optics
The GeneXpert® System utilizes real-time
polymerase chain reaction (PCR) to amplify and
detect target DNA. The system is the first to
fully automate and integrate all the steps
required for PCR-based DNA testing: sample
preparation, DNA amplification and detection.
Self Contained Cartridges
Integrated sample preparation - for automated and
self-contained testing, features and benefits:
- Reagent containment and delivery
- Sample and reagent aliquoting and mixing;
continuous flow for large volume handling
- Cell separation and concentration using
modular filter and solid-phase holder assemblies
- Rapid cell lysis using ultrasonic
techniques
- DNA or RNA capture, enrichment,
purification using chips or solid-phase
materials
- Preparing reaction mixture and filling
integrated PCR reaction tube
- The cartridges are designed for use with
Cepheid's real-time modules, thus fully
integrating the steps associated with DNA
extraction, amplification, and detection in one
system and one procedure.
The Mx™ Family of qPCR
Systems from Stratagene offers the highest
performance and flexibility in qPCR Systems at the
most affordable price. The Mx3000P® qPCR System is
not just an entry-level QPCR system, but is a
high-performance, full-featured instrument system
designed to accommodate most experimental designs.
The new Mx3005P™ qPCR System advances the proven
Mx3000P system by offering unmatched flexibility and
capability to support more real-time qPCR
applications and chemistries. The flexible Mx3005P
system will accommodate your research needs now and
in the future.
Mx3000P® qPCR System
Cited in more than 3000
publications, the Agilent Mx3005P and Mx3000P QPCR
Systems are the most flexible—and
reliable—instruments available for gene expression
analysis, microarray data validation, SNP
genotyping, pathogen detection, DNA methylation
analysis, and chromatin immuno-precipitation
studies. Agilent’s qPCR software, MxPro, provides
users with an intuitive interface, quick experiment
design, powerful data analysis and easy report
generation. All of these features and more make qPCR
with the Mx instrument an exciting and dependable
user experience.
Mx3005P™ qPCR System
Cited in more than 3000
publications, the Agilent Mx3005P and Mx3000P QPCR
Systems are the most flexible—and
reliable—instruments available for gene expression
analysis, microarray data validation, SNP
genotyping, pathogen detection, DNA methylation
analysis, and chromatin immuno-precipitation
studies. Agilent’s qPCR software, MxPro, provides
users with an intuitive interface, quick experiment
design, powerful data analysis and easy report
generation. All of these features and more make qPCR
with the Mx instrument an exciting and dependable
user experience.
MXPro ET QPCR
Software
Mx Pro QPCR software with FDA guideline 21 CFR Part
11 compatible features. Four key elements have been
added to create the ET software version; secure
application login, database file management,
electronic audit trail, and report generation.
Mx3000P®
and
Mx3005P™ qPCR System Features
Instrument
Features:
- Excitation: Quartz tungsten-halogen lamp
via scanning fiberoptics
- Detector: Single photomultiplier tube (PMT)
via scanning fiberoptics
- Filters: Customizable filter wheels with
four (Mx3000P® system) or five (Mx3005P™ system)
user-selected filter sets
- Filter control: Custom filter path
selection to support FRET signal detection
(Mx3005P™ system only)
- Thermal system: Solid state, Peltier-based,
96-well block thermal system
- Sample format: Standard 96-well plates,
8-strip tubes, 200-µl tubes, optimized for 25-µl
reactions
- Embedded computer collects and saves
experiment data during power failure or computer
crash
- Dimensions: 13" (33 cm) W x 18" (46 cm) D x
17" (43 cm) H, Weight: 45 lbs. (20.4 kg)
- Power: 100 to 230 VAC +/- 10%, 50/60 Hz
System Performance:
- Linear dynamic range: 10 orders of
magnitude, standard curve method
- Excitation range: 350 to 750 nm
- Emission range: 350 to 700 nm
- Temperature uniformity: +/- 0.25°C at 72°C
- Thermal block ramp rate: Up to 2.5°C/second
- Amplification rate: Standard 40-cycle,
two-step QPCR reaction completed in 90 minutes;
reaction is completed in 60 minutes using
FullVelocity™ SYBR® Green QPCR reagents (without
dissociation curve)
- Discrimination: Distinguish samples with
5,000 and 10,000 template copies with a 99.7%
confidence level
- Detection down to single-copy equivalents
MXPro ET QPCR Software
- Automatically detects and interfaces with
Mx3000P (4-color) or Mx3005P system (5-color)
system
- Interfaces with Mx3000P and Mx3005P systems
simultaneously and supports up to six systems in
any combination attached to a single computer
- Streamlined plate setup with dye editing
dropdown menu
- Right mouse-click capability throughout the
software to allow quicker and easier access to
menu functions
- The Comparative Quantification module
generates and displays normalized fold change
values on a chart using a base 2 logarithmic
scale.
- Gene expression fold change is displayed
with upper and lower limit error bars based on
replicate variability for up to four targets
normalized to a single reference gene with
efficiency corrections for each gene target
- View amplification plots and standard
curves for up to five targets on the same screen
with threshold changes instantly updated on the
standard curve
- Improved text reports display
target-specific data analysis parameters at the
top of each report and allow for customization
through adding/removing columns and enhanced
sorting capability
- Export any data set directly into .xls,
.ppt, .bmp, .txt, or .xml format. Charts and
graphs can be exported directly to Microsoft®
PowerPoint® and Excel®
- Auto generation of charts and graphs in
Excel during export
- Create custom consolidated data reports by
selecting which analysis screens to include and
how the report will organize the data
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