Comparative Specifications for All Bio-Rad Real-Time PCR Detection Systems

Feature	CFX Connect	CFX96 Touch	CFX384 Touch
Excitation range	450–535 nm	450–684 nm	450–650 nm
Light source	3 LEDs in optics shuttle	6 LEDs in optics shuttle	5 LEDs in optics shuttle
Optical detection	3 photodiodes	6 photodiodes	5 photodiodes
Detection range	515–580 nm	515–730 nm	515–690 nm
Multiplex capability	Up to 2 targets	Up to 5 targets	Up to 4 targets
Gradient range	30–100°C	30–100°C	30–100°C
Maximum gradient span	24°C	24°C	24°C
Maximum ramp rate	5°C/sec	5°C/sec	2.5°C/sec
Dimensions (W x D x H)	33 x 46 x 36 cm	33 x 46 x 36 cm	33 x 46 x 36 cm
Weight	21 kg	21 kg	21 kg
Base thermal cycler	CFX Connect	C1000 Touch	C1000 Touch

The CFX96 Touch real-time PCR detection system builds on the power and flexibility of the C1000 Touch thermal cycler to create an exceptional real-time PCR system. Its unsurpassed thermal cycler performance plus innovative optical design produce accurate, reliable data. The powerful yet intuitive CFX Manager software accelerates every step of your real-time PCR research, shortening the time between getting started and obtaining great results.

• Quickly set up runs and monitor amplification traces in real time on the integrated LCD touch screen
• Get results right away – quick installation and factory calibrated optics let you set up the system in seconds
• Fit experiments into your schedule – fast thermal cycling produces results in less than 30 minutes
• Save research time – thermal gradient feature lets you optimize reactions in a single experiment
• Minimize sample and reagent usage – perform up to five-target multiplexing using small sample volumes
• Rely on performance – innovative technology with long-lasting LEDs and solid-state components provides maximum reliability and optimal quantitative results
• Analyze results when and where you want – receive email notification with an attached data file when a run is finished
• Configure the system to fit your laboratory needs – run without a computer, run up to four instruments from one computer, or integrate with the CFX automation system for higher throughput
• Run high resolution melt (HRM) experiments on the CFX96 Touch or CFX384 Touch system and perform data analysis using seamless data import to Precision Melt Analysis™ software

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With the CFX96 system, you can:

• Get great results right away — quick installation and factory-calibrated optics let you set up the system in seconds
• Fit experiments into your schedule — fast thermal cycling produces results in <30 minutes
• Save research time — thermal gradient feature lets you optimize reactions in a single experiment
• Minimize sample and reagent usage — reliable results obtained with sample volumes as low as 10 μl
• Rely on performance — innovative technologies with long-lasting LEDs and solid-state components provide maximal reliability and optimal quantitative results
• Analyze results when and where you want — software can send e-mail notification with attached data file when the run is finished
• Easily expand your throughput when you need to — up to 4 instruments can be controlled by a single computer

Six-Channel Optics Shuttle

The CFX96 system's solid-state optical technology (six filtered LEDs, each with a corresponding filtered photodiode) maximizes fluorescence detection for specific dyes in specific channels, providing sensitive detection for precise quantitation and target discrimination. Scanning just above the sample plate, the optics shuttle individually illuminates and reads fluorescence from each well with high sensitivity and no cross talk. At every position and with every scan, the optics shuttle is reproducibly centered above each well, so the light path is always optimal and there is no need to sacrifice data collection in one of the channels to normalize to a passive reference.

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CFX384 Touch™ Real-Time PCR Detection System

The CFX384 Touch real-time PCR detection system builds on the power and flexibility of the C1000 Touch™ thermal cycler to create a high-throughput real-time PCR system. With unrivaled stand-alone functionality, innovative optical design, and unsurpassed thermal cycler performance, the CFX384 Touch shortens the time between getting started and obtaining great results.

Features include:

• qPCR that stands alone – quickly set up runs and monitor amplification traces in real time on the large integrated LCD touch screen
• Generate robust results right away – quick installation and factory calibrated optics let you set up the system in seconds
• Rely on performance – innovative technology with long-lasting LEDs and solid-state components provides maximum reliability and optimal quantitative results
• Minimize sample and reagent usage – 4-target multiplex experiments yield optimal results using sample volumes as low as 3 μl
• Analyze results when and where you want – receive email notification with an attached data file when a run is finished
• Save research time – thermal gradient feature lets you optimize reactions in a single experiment
• Configure the system to fit your laboratory needs – run without a computer, run up to four instruments from one computer, or integrate with the CFX automation system for higher throughput
• Trust your results – confirm system performance and data reliability with internal qualification, internal reference spots, system test software, and IQ/OQ. Integrate the CFX384 real-time PCR detection system with good laboratory practice standards for data collection and analysis with CFX Manager™ software, Security Edition

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CFX Connect™ Real-Time PCR Detection System

The CFX Connect real-time PCR detection system incorporates industry-leading optics with a Peltier-based reaction block for precise and reliable qPCR data acquisition. The system offers an integrated solution for all your SYBR^®Green/EvaGreen and duplex experiments. A thermal gradient is easily generated across the 96-well reaction block to support rapid optimization of real-time PCR assays. The CFX Connect system includes the powerful CFX Manager softwarefor system operation, experiment setup, and data analysis.

Features include:

• Easy startup – obtain great results right away with factory calibration, quick setup, and intuitive software
• Effortless optimization – save time and reduce costs optimizing assays in a single run using the thermal gradient
• Powerful data analysis tools – quickly and accurately validate and analyze your data with the advance analysis modules of CFX Manager software
• Accelerate publication submission – include MIQE annotations and generate RDML files using Biogazelle’s qbasePLUS software
• Run high resolution melt (HRM) experiments on the CFX Connect and perform data analysis using seamless data import to Precision Melt Analysis software

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The new MyiQ real-time PCR detection system offers an affordable alternative for the detection of common green fluorescent dyes such as FAM and SYBR Green I. This system interfaces directly with the iCycler thermal cycler, offering superior features such as thermal gradient and Peltier-effect driven performance. The MyiQ real-time PCR detection system is a perfect solution for those just getting started with this technology as well as those looking for additional instruments to handle increasing routine assay demands. This system was developed by the same experts at Bio-Rad who have pioneered performance in real-time PCR. The MyiQ delivers the same excellent-quality data as the iCycler iQ real-time detection system.

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The iQ5 real-time PCR detection system

The iQ5 real-time PCR detection system offers a fresh design for Bio-Rad's new generation of real-time PCR instrumentation. Like its predecessors, the iCycler iQ and MyiQ systems, the iQ5 optical system is a modular upgrade to the iCycler thermal cycler with 96-well reaction block. This powerful new system offers five-target analysis capabilities for multiplex PCR, coupled with a completely redesigned software package.

The iQ5 optical system software offers a full suite of enhanced data collection and data analysis options, including tools for gene expression analysis that measure relative quantities without a standard curve. Regardless of your PCR application, you will find that the iQ5 real-time PCR detection system is the ultimate system for value and flexibility.

Key Features

• Multiplexing of up to 5 fluorophores in each reaction vessel conserves precious samples and reagents
• Real-time PCR thermal gradient enables simple, rapid assay optimization
• Intuitive software for protocol setup and data analysis shortens time to results
• Diverse data analysis and presentation options provide a comprehensive view of your assay results
• Advanced gene expression analysis methods with publication-quality statistics and graphs for relative quantitation studies
• Flexible allelic discrimination module allows easy-to-use, automatic sample identification in genotyping experiments
• Embedded tools for end-point fluorescence analysis enables simple qualitative analysis of sample abundance in single- or multicolor assays
• Robust, reliable thermal cycling performance delivered by the iCycler thermal cycler

Chromo4™  Four-Color Real-Time Detector  (Bio-Rad)

The Chromo4 unit is a real-time detector that can be added to a DNA Engine® cycler

as readily as any of our interchangeable sample blocks.

 

The MJ Research® Chromo4 detector easily converts any DNA Engine thermal cycler into a real-time system, by simply swapping with any of our user-changeable sample blocks. The small size and flexibility of this instrument allow it to keep pace with the evolving needs of the laboratory.    Product Specifications

Key Features:

• Interchangeable detector fits standard DNA Engine base units, new or existing.
• The Chromo4 detector slides into the base in just seconds without the need for tools.
• Swappable Chromo4 photonics shuttle can be ordered with customized filter sets.
• Multiplex with up to four colors. All 96 wells are independently excited and detected for each color to minimize crosstalk.
• Compact size and broad dynamic range make this one of the smallest, most sensitive real-time systems.
• DNA Engine thermal cycler offers precise thermal control and a temperature gradient feature.

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Opticon 2 - Continuous Fluorescence Detection System

Multicolor capability allows detection of SYBR^® Green I and FAM in the first channel, and a range of fluorophores in the second channel — including TET, HEX, VIC, and TAMRA — for a multitude of applications such as RT-qPCR and allelic discrimination

• Product Specifications
• DNA Engine^® thermal cycler offers precision thermal control and a temperature gradient feature permits simultaneous incubation at 12 different temperatures to optimize reactions in a single run.
• Real-time results allow for plotting of signal intensity vs. cycle number and graphically monitoring the thermal profile during the run.
• Extraordinary sensitivity permits reliable detection of one initial template copy, while delivering a linear range of up to ten orders of magnitude in starting copy number (with appropriate chemistry).
• Researcher-oriented software allows quantification of samples and generation of melting curves to verify product identity.
• Innovative optical system incorporates an array of 96 LEDs for excitation and a pair of sensitive PMTs for detection in a robust, no-moving-part design.
• High sample capacity accommodates up to 96 samples in standard, low-profile microplates or strip tubes making specialized disposables unnecessary.
• A compact footprint (34cm wide x 47cm deep x 60cm high) ensures that the Opticon system comfortably fits on any lab bench.

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The MiniOpticon uses illumination technology similar to that of the widely used Opticon™ 2 system—samples are sequentially illuminated by a fixed array of 48 blue-green light-emitting diodes (LEDs). Each LED beam is precisely focused onto its corresponding well to ensure minimal crosstalk and light scattering. Crosstalk is further reduced by sequentially illuminating and detecting each well. LEDs provide a long-lived, maintenance-free excitation source, for years of uninterrupted performance. Emitted fluorescence is detected by one of two filtered, sensitive photodiodes.  This innovative no-moving-parts design allows accurate detection in a compact and robust package for real-time PCR applications and gel-free DNA analysis.  Product Specifications