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 StepOne & StepOnePlus Real-Time PCR System: Easy to use for all levels of experience |
 7500 Real-Time PCR System: Trusted performance & dye - versatility |
 ViiA™ 7 Real-Time PCR System: Powerful features & intuitive design |
 QuantStudio 12K Flex Real-Time PCR System: Maximum throughput, flexibility and scalability |
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| StepOne™ StepOnePlus™ systems |
7500 Fast system | 7500 system | ViiA™ 7 system | QuantStudio™ 12K Flex System | |
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| Price | $ / $$ | $$ | $$ | $$$ | Varies |
| Speed |  |
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| Colors | 3 / 4 | 5 | 5 | 21 | 21 |
| Ease of Use | |
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| Available
blocks |
48 / 96-well | Fast 96-well | 96-well | 96/Fast 96/384-well & TaqMan® Array Card | 96/Fast 96/384-well & TaqMan® Array Card and 3,072 through-hole OpenArray® plate |
| Throughput | / |
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real-time
PCR cyclers (1) On
this page the most prominent real-time PCR cycler
are described. In the cycler descriptions the
specifications and the advantages of the displayed
systems are shown. Which real-time platform meets
your requirements best, depends on your research
application. Some of the systems are designed for
research with low capacities and others are for
high-throughput applications, most in combination
with pipetting robots. A lot of the displayed
cyclers use solid-block for thermal cycling, other
use hot- and cooled-air. Most differences are
obviously in the application software, especially
in the way of data analysis and how the derived
cycle of quantification (Cq is MIQE compliant)
crossing points (CP) or threshold levels (Ct) are
computed. Each of these systems employs either one
of several general types of fluorescent probes for
detection. There are also big differences how data
are displayed and reported. Some of the
limitations of end-point detection in (RT-) PCR
have been assuaged in real-time PCR systems, a
number of which are now on the market. These
systems offer many general technical advantages,
including reduced probabilities of variability and
contamination, as well as online monitoring and
the lack of need for post reaction analyses.
Further, some of these systems were developed with
contemporary applications such as quantitative
PCR, multiplexing, HRM (high resolution melting)
and high-throughput analysis in mind. Initial
template levels can be calculated by analysing the
shape of the curve or by determining when the
signal rises above some threshold value.
A hopefully complete list of the commercially available real-time PCR systems are overviewed on this page and/or summarized below:
WEBINAR --
Characterizing the Performance of qPCR Instruments
– Approaches for Assessment and Comparison
The breadth of
instruments available for quantitative PCR (qPCR)
has continued to grow in the past 5-10 years. With
older platforms now being retired and an abundance
of new technologies available to replace them, lab
managers, technicians, and researchers will need to
effectively compare and evaluate the performance of
these platforms. While new features such as
multiplexing, microfluidics, and integration with
liquid handling automation have enabled higher
throughput and lower operating costs, it has made it
increasing complex to readily compare different
types of instruments and their respective
performance characteristics.
As the list of
features and specifications grows, understanding
some of the key metrics of instrument performance
will become critical for evaluating platforms that
will best meet the needs of a laboratory’s
application focus and assay requirements.
Unfortunately, instrument vendors have not
consistently conformed to any particular standards
for defining and assessing performance
characteristics of qPCR instruments and rarely have
the methods been adequately documented in the
product literature.
In this
webinar, we will define several key performance
metrics of qPCR instruments such as dynamic range,
Cq uniformity, sensitivity, and resolution, and
further discuss their importance in practical terms.
Using data from characterization and verification
studies performed on the IntelliQube instrument from
Douglas Scientific, we will also review approaches
to evaluating these metrics, including assays and
software tools that streamline the analysis and
interpretation of performance testing results.
ViiA7, OpenArray, StepOne & StepOnePLus 7300, 7500, 7500 FAST, 7900HT Real-Time PCR System www.lifetechnologies.com real-time PCR cycler portfolio (PDF) The StepOne™ Real-Time PCR System combines plug and play convenience and uncompromising performance that is tailor-made for both first-time users and real-time PCR experts. By utilizing the full range of TaqMan® gene expression and genotyping assays, the StepOne System delivers the kind of high-quality results you've come to expect from the leader in real-time PCR. The StepOne System provides the power and flexibility experienced users demand, while its intuitive software guides new users through experimental design, operation, and analysis. Part of an elegant, optimized system solution for real-time PCR that includes software, reagents, and instrumentation, the StepOne System delivers remarkable performance with remarkable ease-of-use, at a price that can only be described as...remarkable.
StepOnePlus™ Real-Time
PCR System
read
more
download
StepOnePlus brochureThe Applied Biosystems® StepOnePlus™ Real-Time PCR System, makes it simple and easy to get high-quality real-time PCR results. This remarkably simple 96 well, 4 color, real-time PCR system is designed with a user-friendly, yet powerful, interface for researchers of all experience levels.
Remarkably Simple System The StepOnePlus™ system brings advanced real-time PCR technology to a new level of accessibility. Beginning at the StepOnePlus software homepage, you can seamlessly navigate through all aspects of the real-time PCR method including sample and reaction set-up, thermal cycling, and fluorescent detection. Focused application software analyzes and interprets experimental results. Depending on the experimental design, the system can even help you select and order real-time PCR reagents online by means of convenient links in the Design Wizard. Simply Remarkable Results - Because the StepOnePlus system is factory-calibrated for optical and thermal accuracy, simply remarkable real-time PCR results are available right out of the box. It can discriminate between 2 populations of 5,000 and 10,000 template copies of a TaqMan® Assay with 99.7% confidence. Applications and Software Analysis The StepOnePlus System supports any real-time PCR application.
Instrument software for the Applied Biosystems® StepOne Real-Time PCR System runs on the Windows XP® operating system and provides instrument control, data collection, and data analysis. This user-friendly and intuitive software package includes the following features:
The
7500
Fast Real-Time PCR System
The Applied Biosystems 7500 FAST Real-Time PCR System is a versatile, leading edge platform offering enhanced performance capabilities and a future upgrade to high speed thermal cycling.
The ViiA™ 7 Dx Instrument puts high-productivity qPCR at your fingertips. With the new OptiFlex™ optical system and 4 interchangeable block formats from 96-well to 384-well to TaqMan® Array Micro Fludic Card, the ViiA™ 7 is available for in-vitro diagnostics use. Key product features:
Ideal for performing medium- to high-throughput real-time PCR, the ViiA™ 7 Dx Instrument enhances your lab’s productivity.
The ViiA™ 7 Dx Instrument features the new OptiFlex™ System, which provides enhanced fluorescence detection.
ViiA™ 7 Software ViiA™ 7 software drives value in a number of ways, offering:
QuantStudio® real-time PCR & digital PCR systems Flexibility. Versatility. Speed. Precision. Everyone’s needs are unique and that’s why we have expanded the QuantStudio® family of real-time PCR & digital PCR systems. Now you can pick the qPCR platform that best fits your research requirements—find your fit today. The QuantStudio® 6 Flex Real-Time PCR System is ideal for laboratories with multiple applications and end users on a limited budget. With a planned upgrade path to a QuantStudio® 7 Flex System that accommodates automation or TaqMan® Array Cards, the QuantStudio® 6 Flex System is an ideal qPCR platform to accommodate changing future needs. The QuantStudio® 7 Flex Real-Time PCR System delivers the proven reliability, sensitivity, and accuracy of the ViiA™ 7 System in a new industrial design. The QuantStudio® 7 Flex System has been optimized to enable the broadest range of quantitative PCR applications, with additional dyes, formats, and automation options. Combining flexible throughput capabilities with a streamlined workflow, the QuantStudio® 12K Flex system takes you from targeted discovery through confirmation and screening, all on a single platform. Increase your throughput by as much as 32-fold by running 4 OpenArray® plates in a single run on the QuantStudio® 12K Flex system. It's the equivalent of 32 traditional 384-well qPCR plates or 12,000 data points. When equipped with the OpenArray® block, a single 9700 thermal cycler and the QuantStudio® 12K Flex OpenArray® AccuFill™ System, the QuantStudio® 12K Flex system can produce up to 110,000 data points or more in an eight-hour day. For those that need the greatest throughput, add the QuantStudio™ 12K Flex Automation Robot and multiple 9700 thermal cyclers, and you can achieve over 2,000,000 data points in a day. Start a 12,000 data point experiment typically in 20 minutes or less. Now, you can go beyond the limits of real-time PCR with the new QuantStudio® 3D Digital PCR System. A simple and affordable platform, the QuantStudio® 3D Digital PCR System produces absolute quantification data with the precision and sensitivity you need for your more demanding applications. The QuantStudio® 3D Digital PCR System uses a sealed chip technology, providing a streamlined, reliable, and robust method for performing digital PCR. An affordable platform where you can just load and go—digital PCR is now absolutely attainable.
 The LightCycler™ from Roche Molecular
Biochemicals of Indianapolis harnesses light
technology licensed from Idaho Technology Inc. of
Idaho Falls, and then adds some additional bells and
whistles, such as a built-in microvolume
fluorimetric detection system that enables real-time
quantitative PCR. This system employs thin-walled
glass capillary tubes, and typical PCR experiments
can be performed in less than 30 minutes. Currently,
this system supports two fluorescence-based methods
for the detection of amplification products: the
"general" DNA stain SYBR Green I (a product of
Molecular Probes of Eugene, Ore.), or
sequence-specific hybridization probe pairs. SYBR
Green I exhibits very little fluorescence when free
in solution; emission is greatly enhanced when it
binds to the minor groove of the DNA double helix.
Prior to amplification, the reaction mixture
contains the denatured DNA, the primers, and the
dye. The low-level background fluorescence signal
generated by the unbound dye molecules is subtracted
during computer analysis. After annealing of the
primers, a few dye molecules can bind to the double
strand. During elongation, more and more dye
molecules bind to the newly synthesized DNA,
resulting in dramatically increased light emission.
If the reaction is monitored continuously, this
increase in fluorescence can be viewed in real time.
After denaturation of the DNA during the next
heating cycle, the dye molecules are released and
the fluorescence signal falls. A fluorescence
measurement is performed at the end of the
elongation step of every PCR cycle to monitor the
increasing amount of amplified DNA. The
hybridization probe format employs two specially
designed, sequence-specificoligonucleotides lab-eled
with fluorescent dyes. One oligonucleotide probe
carries a fluorescein label at its 3' end; the
other probe carries a different label (LC Red 640 or
LC Red 705) at its 5' end. The chemical nature of
the hybridization probes prevents their extension:
one probe contains fluorescein at the 3' end,
whereas the 5'-labeled probe contains a 3' phosphate
moiety. The sequences of the two oligonucleotides
are selected so that they hybridize to the amplified
DNA fragment in a head-to-tail arrangement. When the
oligonucleotides hybridize in this orientation, the
two fluorescent dyes are positioned in close
proximity to each other. The first dye (fluorescein)
is excited by the LightCycler's light emitting diode
(LED) filtered light source, and emits green
fluorescent light at a slightly longer wavelength.
When the two dyes are in close proximity, the
emitted energy excites the dye attached to the
second hybridization probe, which subsequently emits
red fluorescent light at an even longer wavelength.
This energy transfer, referred to as fluorescence
resonance energy transfer (FRET), occurs efficiently
only when the dyes are in close proximity (a
distance between 1-5 nucleotides). Thus, in this
type of assay, fluorescent intensity measurements
are made after the annealing steps. The increasing
amount of emitted fluorescence is proportional to
the increasing amount of DNA generated during the
linear phase of the ongoing PCR process.
The
LightCycler
480 Real-Time PCR System is a fully integrated
multiwell-plate based real-time PCR platform for
highly accurate qualitative and quantitative
detection of nucleic acids. Building on the benefits
of Roche's capillary-based LightCycler® Systems, it
goes one step further in offering enhanced
throughput, compatibility with automation equipment
and maximum flexibility regarding hard- and
software.
Providing novel ways to combine speed and accuracy without compromises, the LightCycler® 480 Real-Time PCR System meets the needs of a broad range of applications in research fields such as gene expression studies, discovery and analysis of genetic variation or array data validation. LightCycler® 480 Real-Time PCR System benefits SPEED & ACCURACY
VERSATILITY & CONVENIENCE
COMPATIBILITY & SECURITY
Gene Scanning by High Resolution Melting Curve Analysis generally requires the use of:
In a Gene Scanning experiment, sample DNA is first amplified via real-time PCR in the presence of a proprietary saturating DNA dye. A melting curve is then performed using high data acquisition rates, and data are finally analyzed using a Gene Scanning Software, by three basic steps:
 High-resolution melting curve analysis on the LightCycler 480 PCR system (presented by Roche Aplied Science) Roche Applied Science´s LightCycler® family of real-time PCR systems offer fast, accurate and versatile platforms for genetic variation research. The new plate-based LightCycler® 480 System provides the temperature homogeneity and optical characteristics required for high-performance melting-curve analysis (MCA). On the level of data acquisition and available detection channels, this new instrument opens the way to more advanced applications in the emerging field of gene scanning where amplicons can be screened for unknown sequence variations with low efforts in time and cost. The LightCycler® 480 real-time PCR system: a versatile platform for genetic variation research Real-time PCR is a well established technique for studying genetic variation using various probe-based methods for genotyping as well as high-resolution analysis of whole amplicons melted in the presence of saturating DNA dyes. The latter, relatively new, method allows screening for unknown mutations or DNA modifications. The LightCycler® 480 real-time PCR system is a multiwell plate–based instrument that provides integrated applications for detecting and characterizing genetic variation using all these methodological approaches. Transfering PCRs to HRM-assays on the LightCycler 480 System- Examples for BRCA1 High-resolution melting curve analysis (hrMCA) is an attractive technique to scan for unknown mutations in genes. To evaluate how easy or difficult it is to design hrMCA assays using the LightCycler® 480 Instrument, we selected 3 different fragments in exon 11 of the BRCA1 gene, designed an MCA assay, and tested its sensitivity to detect known variants. Rapid high-throughput Methylation analysis using the LightCycler 480 system (presented by Roche Aplied Science) Large-scale, PCR-based gene analysis can only be performed if signals are generated, captured and analyzed in a highly reproducible manner. More precisely, thermal control, optical readout and the algorithms used to characterize genes qualitatively and quantitatively have to be highly precise and reliable. The LightCycler® 1536 System from Roche Applied Science meets these requirements. The novel platform, which utilizes a proprietary 1536-well plate, is capable of performing high-speed, qPCR-based DNA/RNA analyses in an array-like format. High Throughput — Redefined Break through to a new era in high-throughput real-time PCR with the powerful LightCycler® 1536 System, precisely engineered for miniaturization and parallelization.
LightCycler Nano system - Serious PCR. So much Fun! A small yet complete system with all features you've come to expect from LightCycler® Instruments 1. The LightCycler® Nano Instrument produces high target specificity with high sensitivity characteristic of all LightCycler® Systems. 2. Fast protocols use state-of-the-art, 32-well thermal cycler technology for excellent thermal precision. 3. Robust solid-state optics provide complete spectral information. Learn more about LightCycler® Nano System features Sleek, built to enjoy in every lab, on every bench 1. Small enough for plenty of space on your benchtop. 2. Experience ease-of-use with flexible workflows. 3. Easily install using plug and play. 4. So quiet. You won't know it's running if it wasn't for the light show. 5. Intuitive software from assay setup to analysis. See the LightCycler® Nano System Gallery Great looks meets fast, sensitive and accurate qPCR 1. The LightCycler® Nano System embodies experimental freedom. 2. The instrument uses standard 8-tube PCR strips that come with caps. 3. Flexible reaction volumes permit different levels of sensitivity. 4. Proven reagents for SYBR Green or probe-based assays are available. 5. Use different methods for quantification and mutation analysis, including HRM. 6. Use your preferred operating system, Mac OS or Windows, or go standalone with a USB flash drive. See the LightCycler® Nano System performance data LightCycler 96
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Mastercycler® ep realplex - real-time PCR solutions
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