Development of a new set of reference genes for normalization of real-time RT-PCR data of porcine backfat and longissimus dorsi muscle, and evaluation with PPARGC1A
Tim Erkens1, Mario Van Poucke1, Jo Vandesompele2, Karen Goossens1, Alex Van Zeveren1 and Luc J Peelman*1
Address: 1Department of Nutrition, Genetics and Ethology, Faculty of Veterinary Medicine, Ghent University, Heidestraat 19, 9820 Merelbeke, Belgium and 2Centre for Medical Genetics, Ghent University Hospital, De Pintelaan 185, 9000 Ghent, Belgium
BMC Biotechnology 2006, 6:41

Selection of reference genes for quantitative real-time PCR in bovine preimplantation embryos.
Karen Goossens1, Mario Van Poucke1, Ann Van Soom2, Jo Vandesompele3,
Alex Van Zeveren1 and Luc J Peelman*1
Address: 1Department of Animal Nutrition, Genetics, Breeding and Ethology, Faculty of Veterinary Medicine, Ghent University, Heidestraat 19, B-9820 Merelbeke, Belgium, 2Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium and 3Center for Medical Genetics Ghent, Ghent University Hospital, Medical Research Building, De Pintelaan 185, B-9000 Ghent, Belgium
BMC Developmental Biology 2005, 5: 27

Universal reference method for real-time PCR gene expression analysis of preimplantation embryos.
Neil Ivan Bower, Ralf Joachim Moser, Jonathan Robert Hill, and Sigrid Arabella Lehnert
BioTechniques 42:199-206 (February 2007)

Development and evaluation of canine reference genes for accurate quantification of gene expression.
Bas Brinkhof *, Bart Spee, Jan Rothuizen, Louis C. Penning
Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, 3508 TD Utrecht, The Netherlands
Analytical Biochemistry (2006)

Normalizing genes for quantitative RT-PCR in differentiating human intestinal epithelial cells and adenocarcinomas of the colon.
Anders Bondo Dydensborg, Elizabeth Herring, Joe¨lle Auclair, Eric Tremblay, and Jean-Francois Beaulieu
Canadian Institutes of Health Research Group in Functional Development and Physiopathology
of the Digestive Tract, De´partement d’Anatomie et de Biologie Cellulaire, Faculte´
de Me´decine, Universite´ de Sherbrooke, Sherbrooke, Que´bec, Canada
Am J Physiol Gastrointest Liver Physiol 290: G1067–G1074, 2006.

Statistical Selection of Maintenance Genes for Normalization of Gene Expressions.
Yifan Huang Jason C. Hsu† Mario Peruggia‡ Abigail A. Scott
Statistical Applications in Genetics and Molecular Biology Volume 5, Issue 1 2006 Article 4

Evaluation of Reference Genes for Studies of Gene Expression in Human Adipose Tissue.
Britt G. Gabrielsson,* Louise E. Olofsson,* Anders Sjo¨gren,‡ Margareta Jernås,* Anna Elander,†
Malin Lo¨nn,* Mats Rudemo,‡ and Lena M. S. Carlsson*
OBESITY RESEARCH Vol. 13 No. 4 2005 649

A Relevant Reference Gene and Normalization for mRNA Real-Time PCR Quantification in Specimens with Distinct Cell Types and Variant Integrity.
Sugahara Kazunori et. al.
1Department of Laboratory Medicine, Nagasaki University Graduate School of Biomedical Sciences
Acta medica Nagasakiensia Vol.51, No.2(2006) pp. 57-63

Quantification of cDNA generated by reverse transcription of total RNA provides a simple alternative tool for quantitative RT-PCR normalization.
Jirˇí Libus and Helena Štorchová
Institute of Experimental Botany, Prague, Czech Republic
BioTechniques 41:156-164 (2006)

Error propagation in relative real-time reverse transcription polymerase chain reaction quantification models: The balance
between accuracy and precision.

Oddmund Nordga˚rd a,*, Jan Terje Kvaløy b,c, Ragne Kristin Farmen a,1, Reino Heikkila¨ a
a Department of Hematology and Oncology, Stavanger University Hospital, 4068 Stavanger, Norway
b Department of Mathematics and Natural Science, University of Stavanger, 4036 Stavanger, Norway
c Division of Research and Human Resources, Stavanger University Hospital, 4068 Stavanger, Norway
Analytical Biochemistry 356 (2006) 182–193

QuantitativeMulti-Gene Expression Profiling of Primary Prostate Cancer.
Uta Schmidt,1 Susanne Fuessel,1 Rainer Koch,2 Gustavo B. Baretton,3
Andrea Lohse,1 Silke Tomasetti,1 Susanne Unversucht,1 Michael Froehner,1 Manfred P. Wirth,1 and Axel Meye1*
1Department of Urology,Technical University of Dresden,Germany
2Institutes ofMedical Informatics and Biometry,Technical University of Dresden,Germany
3Institute of Pathology,Technical University of Dresden,Germany
The Prostate 66:1521^1534 (2006)

Selection of reference genes for quantitative RT-PCR studies in striped dolphin (Stenella coeruleoalba) skin biopsies.
Giacomo Spinsanti1§, Cristina Panti 1, Elisa Lazzeri1, Letizia Marsili2, Silvia Casini2,
Francesco Frati1 and Maria Cristina Fossi2
 1Evolutionary Biology Department, University of Siena, Via A. Moro 2, 53100 Siena, Italy
2Environmental Sciences Department, University of Siena, Via P.A. Mattioli 4, 53100 Siena, Italy
BMC Molecular Biology 2006, 7:32

Genomic DNA standards for gene expression profling in Mycobacterium tuberculosis.
Adel M. Talaat, Susan T. Howard1, Walker Hale IV, Rick Lyons1, Harold Garner and Stephen Albert Johnston*
Center for Biomedical Inventions and Departments of Medicine, Microbiology and Biochemistry, University of
Texas±Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-8573, USA and
1Department of Internal Medicine, University of New Mexico Health Science Center, 915 Camino de Salud,
Albuquerque, NM 87131, USA
Nucleic Acids Research, 2002, Vol. 30 No. 20 e104

Housekeeping gene selection for real-time RT-PCR normalization in potato during biotic and abiotic stress.
Nathalie Nicot, Jean-Francxois Hausman, Lucien Hoffmann and Danie` le Evers*
Centre de Recherche Public-Gabriel Lippmann, Environment and Biotechnologies Research Unit, 41 rue du Brill,
L-4422 Belvaux, Luxembourg
Journal of Experimental Botany, Vol. 56, No. 421, pp. 2907–2914, 2005

Developmental expression profiles of Xenopus laevis reference genes.
Sindelka R, Ferjentsik Z, Jonak J.
Dev Dyn. 2006 235(3):754-8.


Statistical Selection of Maintenance Genes for Normalization of Gene Expressions.
Yifan Huang Jason C. Hsu† Mario Peruggia‡ Abigail A. Scott
Statistical Applications in Genetics and Molecular Biology (2006) 5(1)1 Article 4

Quantification of cDNA generated by reverse transcription of total RNA provides a simple alternative tool for quantitative RT-PCR normalization.
Jiri Libus and Helena Štorchová
Institute of Experimental Botany, Prague, Czech Republic
BioTechniques 41:156-164 (2006)

Selection of reference genes in mouse embryos and in differentiating human and mouse ES cells.
ERIK WILLEMS1, ILEANA MATEIZEL2, CAROLINE KEMP1, GREET CAUFFMAN2, KAREN SERMON2
and LUC LEYNS*,1
1Lab for Cell Genetics, Vrije Universiteit Brussel (VUB) and 2Research Center for Reproduction and Genetics, University Hospital and Medical School of the Vrije Universiteit Brussel (AZ-VUB), Brussels, Belgium
Int. J. Dev. Biol. 50: 627-635 (2006)

Equivalence test in quantitative reverse transcription polymerase chain reaction: confirmation of reference genes suitable for normalization
Haller F, Kulle B, Schwager S, Gunawan B, von Heydebreck A, Sultmann H, Fuzesi L.
Anal Biochem. 2004 Dec 1;335(1):1-9.

Genome-Wide Identification and Testing of Superior Reference Genes for Transcript Normalization in Arabidopsis.
Tomasz Czechowski, Mark Stitt, Thomas Altmann, Michael K. Udvardi, and Wolf-Ru¨ diger Scheible*
Max-Planck Institute of Molecular Plant Physiology, 14476 Potsdam, Germany
Plant Physiology (2005) 139, 5–17

Generic normalization method for real-time PCR Application for the analysis of the mannanase gene expressed in germinating tomato seed.
Dimitris Argyropoulos1, Charoula Psallida1 and Caroline G. Spyropoulos2
1 Institute of Biotechnology, National Agricultural Research Foundation, Athens, Greece
2 Department of Botany, Faculty of Biology, University of Athens, Greece
FEBS Journal 273 (2006) 770–777

Sex-dependent expression of seven housekeeping genes in rat liver.
ASHISH S VERMA AND BERNARD H SHAPIRO
Laboratories of Biochemistry, University of Pennsylvania, School of Veterinary Medicine, Philadelphia, PA, USA
Journal of Gastroenterology and Hepatology (2006) 21, 1004–1008

Housekeeping genes in cancer: normalization of array data.
Khimani AH, Mhashilkar AM, Mikulskis A, O'Malley M, Liao J, Golenko EE, Mayer P, Chada S, Killian JB, Lott ST.
Biotechniques. 2005 38(5): 739-745