Reference in qPCR www.Gene-Quantification.info

www.Gene-Quantification.info 21st Anniversary!
The Reference in qPCR & dPCR - Academic & Industrial Information Platform

Founded February 2002 -- Since 21 years, the Gene Quantification platform describes and summarises all technical aspects involved in quantitative gene expression analysis using real-timePCR (qPCR) & digital-PCR (dPCR) & Next Generation Sequencing. It presents the majority of new and innovative qPCR & dPCR applications, chemistries, methods, data analysis algorithms, MIQE & QC strategies, cyclers, kits, dyes, analysis methods, interesting events & workshops, and services involved. Please browse our page directory with all essential keywords or the qPCR platform, a summary of commercial and academic institutions presenting their PCR related tools.
Amplify your knowledge in qPCR, dPCR and NGS! Follow our streaming server presenting 500 talks => eConferences.de

MIQE iBook ... 5th eds		EV & Exosomes & EV RNA / Liquid Biopsy / Biomarker Discovery / Transcriptional Biomarkers
Books eBooks ePub		qPCR Platform / Protocols / Methods / Translation / ASIA portal / GQ Page Directory
eConferences & Webinars		"free access" to 500 recorded talks Gene Quantification Events 2010-2023 / Talks / Webinars
Quantification Strategy		Quantification strategies: absolute Quantification & relative Quantification / qPCR Evolution
Normalisation		RT-qPCR normalisation strategies & microRNA / Genorm / BestKeeper / RefGenes
qPCR & Optimisation		real-time PCR / interesting reviews / MIQE & Apps & iBook / Primer-Recources & Algorithms
Bioinformatics & Statistics		Big Data in Biology / data analysis / Regulatory Networks / data clustering / Integrated Analysis
Software Downloads		REST (main page) / REST-2009 / RDML / RefGenes / GenEx 7.0 ... FREE DOWNLOAD
RNAi -- RNA interference		siRNA / saRNA / microRNA & microRNA normalisation / mirtron / qPCR statistics
Gene Quantification Events		impressions GQ2023 / qPCR dPCR NGS 2023 / MicroGenomics 2023 / eConferences / previous Events
digital PCR & qPCR Array		digital-PCR ... UPDATE / MIQE & dPCR / qPCR array / normalisation and analysis software
Cyclers & Robotics		real-time PCR hardware: overview of qPCR cyclers / qPCR robotics / page statistics
RNA & RT & RNA QC		reverse transcription / mRNA transcript analysis ... UPDATE / RNA QC & RNA / DNA integrity
Dyes & HRM dyes		detection dyes, probes, and chemistries in real-time PCR / HRM dyes / Chips & Lab-on-Chip
Liquid Biopsy & Biomarkers		Exosomes / EV & exRNA ... UPDATE / CNA / microRNA / Transcriptional Biomarkers
Molecular Physiology		CNA / HRM / CNV / microRNA / siRNA / saRNA / RNAi / digital-PCR / single-cell qPCR
qPCR Efficiency		determination of real-time qPCR efficiency by various methods ... new efficiency papers!
qPCR & dPCR applications		EVs & Exosomes / circulating nucleic acids / digital-PCR / single-cell handling / single-cell qPCR
GQ info portal		microRNA / CNV / microRNA transfer / HRM / RNAi / siRNA / saRNA / Liquid Biopsy


	Gene Quantification Newsletter is sponsored by

qPCR platform participating companies:

qPCR platform participating companies in alphabetical order

Analytik-Jena.gene-quantification.info

Analytik Jena offers a wide produt variety of thermal cyclers as well as real-time thermal cyclers.

Qiagen.gene-quantification.info

Qiagen - Sample to insight

Bioron.Gene-Quantification.info

BIORON – Next Generation Tools for Life Science

TATAA.gene-quantification.info

TATAA Biocenter - Amplifying discovery and accelerating development

Bio-Rad.gene-quantification.info

Bio-Rad Laboratories - A complete solution. A focused approach.

NEB.Gene-Quantification.info

New England BioLabs -- Luna® qPCR and RT-qPCR Reagents for rapid, sensitive and precise detection of RNA, DNA and cDNA targets

BioMolecularSystems.Gene-Quantification.info

BioMolecular Systems - Meet Mic. Fast. Accurate. Compact.

Eurogentec.Gene-Quantification.info

Eurogentec - Your one-stop-shop Real-Time PCR supplier

Biozym.Gene-Quantification.info

Biozym - Science is our business

GNA-Biosolutions.Gene-Quantification.info

GNA Biosolutions - Simplified molecular testing in minutes

Bioline.gene-quantification.info

Bioline – The PCR Company

Promega.gene-quantification.info

Promega - Be successful in your qPCR experiments with our robust amplification master mixes

Stilla-Technologies.gene-quantification.info

Stilla Technologies - Naica System Workflow - a fast and simple workflow

LifeTechnologies.gene-quantification.info

Life Technologies - Advanced gene expression services

TIB-molbiol.gene-quantification.info

TIB-molbiol - Our business is DNA.

LGC-Biosearchtechnologies.Gene-Quantification.info

LGC - Biosearch Technologies - Advancing Nucleic Acid Technologies

QuantaBio.gene-quantification.info

QuantaBio is dedicated to perfecting qPCR reagents that deliver superior performance you can rely on.

nexttec.gene-quantification.info

nexttec Biotechnologie GmbH - the fastest way to get PURIFIED DNA - tested millions of time

BioSistemika.gene-quantification.info

BioSistemika - Software development partners for life sciences - Helping deliver your ideas!

PrimerDesign.gene-quantification.info

PrimerDesign - Driving technology, Empowering researchers

LTF.gene-quantification.info

LTF Labortechnik - Results at a glance !

Your want to join the qPCR or eConferences platform! Contact us via qPCRplatform@gene-quantification.info


Cooperating Institutions & Laboratories:
		Express your genius with qPCR The TATAA Biocenter is a research provider that offers training and commissioned services within molecular diagnostics and gene expression analysis using real-time PCR and other molecular techniques to quantify nucleic acids. Our competence is based on knowledge and experience accumulated through years of research at leading European Universities. TATAA offers comprises the entire field of real-time PCR services, including commissioned research, hands-on training, and custom design of real-time PCR assays. Further activities: 1) Open and tailor-made hands-on training courses; 2) Commissioned research and development; 3) Custom design of qPCR assays and assay validation; 4) Product development
		GenEx software - GenEx offers advanced methods to analyze real-time qPCR data with simple clicks of the mouse. The methods are suitable to select and validate reference genes, classify samples, group genes, monitor time dependent processes and much more.
		Prof. Stephen Bustin - Stephen Bustin is Professor of Molecular Medicine at Anglia Ruskin University www.anglia.ac.uk at the Postgraduate Medical Institute.
		Laboratory of Gene Expression Prof. Mikael Kubista´s laboratory of Gene Expressionis at the Institute of Biotechnology (BTU) @ Czech Academy of Science (CAS). The Institute of Biotechnology is member of BIOCEV, CzechBIO, The Technology Centre, Czech Academy of Science (CAS) BIOCEV. His group is developing real-time PCR assays for different applications such as routine clinical diagnostic and methods for high through put measurements
		GeneCore offers service in the field of single-cell and spatial transcriptomics, with 18 years experience with single-cell profiling. At our state-of-the-art GeneCore service laboratory, they specialize in cutting-edge techniques for RNA library preparation, empowering researchers to uncover the intricate genetic mechanisms behind biological processes. With their long lasting expertise in the RNA analysis, they offer comprehensive analysis of individual cells, enabling a deeper understanding of cellular diversity, heterogeneity and gene expression patterns. The GeneCore laboratory is equipped with advanced spatial transcriptomic technology, allowing researchers to visualize gene expression within intact tissue samples, unraveling the spatial organization of cellular activity. The team takes pride in delivering accurate and reliable results through our meticulous RNA sequencing workflows, ensuring high-quality data for your research needs. Collaborating with leading scientists and utilizing the latest sequencing platforms, our service laboratory is committed to providing exceptional services in the field of RNA sequencing, single cell RNA sequencing, and spatial transcriptomics.
		EMBL GeneCore is the in-house genomics service center at EMBL in Heidelberg, Germany, for DNA sequencing, next-generation-sequecing (NGS), S.A.G.E., microarrays, real-time qPCR and much more ... Services are provided on non-commercial basis for the EMBL community and academic institutions in the context of scientific collaborations within the EMBL member states. Tight links to industry provide state-of-the-art equipment. The experiments and their results feed directly back into improvements of instruments and technologies, spawning the next generation of scientific questions and technologies.
		Biogazelle is the quantitative PCR data-analysis company, founded in 2007 as a Ghent University spin-off by Prof. Jo Vandesompele and Dr. Jan Hellemans. Both founders have more than 15 years of experience in real-time PCR experiment design, assay development and data-analysis. They wrote one of the most influential papers on normalization of gene expression and on data-analysis (together cited more than 10,000 times). Biogazelle's flagship product qbase+ is the most powerful, flexible, and user-friendly real-time PCR data-analysis software based on the proven geNorm and qBase technology, enhanced with proprietary algorithms and time-saving features. qBASE+ is truly accelerating your research.
		geNorm VBA applet for Microsoft Excel determines the most stable reference genes from a set of tested genes and calculates a gene expression normalization factor based on the geometric mean of a user-defined number of reference genes. qBase is a collection of macros for Microsoft Excel for the management and automated analysis of real-time quantitative PCR data. The program employs a delta-Ct relative quantification model with PCR efficiency correction and multiple reference gene normalization.

Media Partner:
		eConferences -- Amplify your knowledge in MDx using qPCR, dPCR and NGS! This streaming portal is dedicated to scientists from the community of qPCR, digital-PCR, Next Generation Sequencing (NGS), MicroGenomics (MG) and Molecular Diagnostics (MDx). You’ll find here all the records from more than 420 presentations held at Gene Quantification, qPCR dPCR & NGS and MG Events in the past years – starting qPCR 2010 in Vienna, over MicroGenomics 2014 in Paris, to GQ2019 in Freising-Weihenstephan. We provide the presentations via streaming technology in high quality – high resolution and perfect sound quality in high speed – on any internet browser or mobile device.
		Technology Networks Technology Networks was established around 15 years ago with the aim of providing informative websites for life science professionals. The team run, update and provide an outstanding web-based information solution for those working within the Life Science and Drug Discovery community. Since its humble beginnings running just one website based on Combinatorial Chemistry. Today Technology Networks has expanded its portfolio to include over 30 communities: e.g. qPCR, Next Gen Sequencing, AgriGenomics, Molecular & Clinical Diagnostics, Genomics, and Genotyping & Gene Expression

www.Gene-Quantification.info is edited by Michael W. Pfaffl The Gene Quantification web pages are educational and academic sites with the only purpose of facilitating access to biology-related information over the internet. © 2002-2023 21st Anniversary version 190.65 last update @ 1st November 2023 Copyrights Disclaimer EU - DSGVO